Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Babesia also called Nuttallia, is an apicomplexan parasite that infects red blood cells and is transmitted by ticks. Babesia is a protozoan parasite found to infect vertebrate animals, mostly livestock mammals and birds, but also occasionally humans. To date, more than 100 Babesia species have been scientifically described, which are specific to many species of mammals and birds.
In dogs, a large form of Babesia spp. is represented by Babesia canis and small forms of the disease are represented by Babesia gibsoni, B. conradae, and B. vulpes. Babesia canis includes three subspecies (B. caniscanis, B. canisrossi, and B. canisvogeli), which have been identified based on their cross-immunity, serological testing, vector specificity, and molecular phylogeny. Small Babesia spp. include B. gibsoni which comes from Asia, B. conradae found in dogs in the western United States, and B. microti-like founded in Spain, renamed Theileria annae, and later B. vulpes. Domestic dogs and cats can be infected with these Babesia species Dogs can have subclinical disease (i.e. no clinical signs) or illness that ranges from mild (e.g. lethargy, reduced appetite) to severe (e.g. pallor and weakness related to anemia). Severe disease can result in death.
Among the various diagnostic techniques used for detection, Real-time quantitative polymerase chain reaction (real-time PCR) assay is one of the sensitive, reliable and desirable methods for specific detection and quantification of causative agents.
Considering of the geographical distribution of Babesia spp. infections in world, we established a series of one-step real-time PCR assays for detection of specific or genus babesia species as shown below. All assays were optimized at a universal thermal cycling condition, and evaluated under monoplex or multiplex condition for detection of babesia DNA, which will be useful in early diagnosis and consequently addressing the threat of babesia agents in dogs.
Pathogens Tested
APB-218 - Babesia species (Excluding Babesia microtigroup)
APB-219 - Babesia gibsoni (Asian genotype) (inclusive of Babesia spp.)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Feline Immunodeficiency Virus (FIV) is a retrovirus that affects cats worldwide. It weakens a cat's immune system over time, leaving them more susceptible to infections and certain cancers. FIV is primarily transmitted through bite wounds, so outdoor cats and those with a history of fighting are at higher risk. It's important for cat owners to be aware of FIV and take preventive measures, such as keeping cats indoors and getting them tested regularly.
Symptoms of cat FIV include poor coat condition, fever that keeps coming back, lack of appetite, inflammation in the mouth and gums,chronic or recurrent infections in the eyes, skin, upper respiratory tract, or bladder, constant diarrhea, persistent eye problems and seizures.
Two sets of primers were designed at a universal thermal cycling condition, and evaluated for detection of FIV RNA by real-time RT-PCR, which will be useful in early diagnosis and consequently addressing the threat of FIV agents in cats.
Method
Real Time RT-PCR
Sample Type
EDTA Blood
Transport Condition
Sample should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Molecular based methods like PCR, Real-Time PCR and more recently next generation sequencing (NGS) have revolutionized the field of veterinary diagnostics.
At MBG, we offer detection of pathogenic viruses, bacteria and parasites using molecular methods, which are fast and highly sensitive to detect microbial
pathogens in various specimens.
MBG is an ISO 17025 accredited facility and benefits from an isolated containment level 3 facility for processing highly (level 3) contagious pathogens.
Babesia also called Nuttallia, is an apicomplexan parasite that infects red blood cells and is transmitted by ticks. Babesia is a protozoan parasite found to infect vertebrate animals, mostly livestock mammals and birds, but also occasionally humans. To date, more than 100 Babesia species have been scientifically described, which are specific to many species of mammals and birds.
In dogs, a large form of Babesia spp. is represented by Babesia canis and small forms of the disease are represented by Babesia gibsoni, B. conradae, and B. vulpes. Babesia canis includes three subspecies (B. caniscanis, B. canisrossi, and B. canisvogeli), which have been identified based on their cross-immunity, serological testing, vector specificity, and molecular phylogeny. Small Babesia spp. include B. gibsoni which comes from Asia, B. conradae found in dogs in the western United States, and B. microti-like founded in Spain, renamed Theileria annae, and later B. vulpes. Domestic dogs and cats can be infected with these Babesia species Dogs can have subclinical disease (i.e. no clinical signs) or illness that ranges from mild (e.g. lethargy, reduced appetite) to severe (e.g. pallor and weakness related to anemia). Severe disease can result in death.
Among the various diagnostic techniques used for detection, Real-time quantitative polymerase chain reaction (real-time PCR) assay is one of the sensitive, reliable and desirable methods for specific detection and quantification of causative agents.
Considering of the geographical distribution of Babesia spp. infections in world, we established a series of one-step real-time PCR assays for detection of specific or genus babesia species as shown below. All assays were optimized at a universal thermal cycling condition, and evaluated under monoplex or multiplex condition for detection of babesia DNA, which will be useful in early diagnosis and consequently addressing the threat of babesia agents in dogs.
Pathogens Tested
APB-218 - Babesia species (Excluding Babesia microtigroup)
APB-219 - Babesia gibsoni (Asian genotype) (inclusive of Babesia spp.)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
Feline Immunodeficiency Virus (FIV) is a retrovirus that affects cats worldwide. It weakens a cat's immune system over time, leaving them more susceptible to infections and certain cancers. FIV is primarily transmitted through bite wounds, so outdoor cats and those with a history of fighting are at higher risk. It's important for cat owners to be aware of FIV and take preventive measures, such as keeping cats indoors and getting them tested regularly.
Symptoms of cat FIV include poor coat condition, fever that keeps coming back, lack of appetite, inflammation in the mouth and gums,chronic or recurrent infections in the eyes, skin, upper respiratory tract, or bladder, constant diarrhea, persistent eye problems and seizures.
Two sets of primers were designed at a universal thermal cycling condition, and evaluated for detection of FIV RNA by real-time RT-PCR, which will be useful in early diagnosis and consequently addressing the threat of FIV agents in cats.
Method
Real Time RT-PCR
Sample Type
EDTA Blood
Transport Condition
Sample should be transported at 4°C.
Turn Around Time (TAT)
Normal Turnaround time for pathogen Identification is within 5 working days. Urgent Samples will be reported within half of the minimum test period & will be Charged Double.
Samples delivered after 11:00 AM will be processed next working day unless urgent.
